transmission electron microscopy  (Hitachi Ltd)

Journal: iScience

Article Title: Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction

doi: 10.1016/j.isci.2026.116268

Figure Lengend Snippet: Structural evaluation of the mouse TM following acute IOP elevation (A) Photographs reveal that mice underwent direct cannulation of the anterior chamber (AC) in the eye. Eyeballs were harvested immediately after the procedure, and the gross appearance is illustrated in the right. (B) Intraocular pressure (IOP) measurement following acute ocular hypertension. IOP was measured at baseline (0 days), 3 days, and 7 days following 45 mmHg pressure for 1 h. Data represent mean ± SD. two-way ANOVA was used for comparing IOP between control and HIOP groups across time points. Post hoc test: ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. control group at corresponding time point. N = 9 mice per group. (C) Representative hematoxylin staining of anterior chamber angles 3 days after IOP elevation. Magnification bars: 1 mm. (D) Ultrastructural appearance of the TM tissues by transmission electron microscopy. Leftmost: representative lower magnification image of the TM and SC. Right: higher magnification of the TM cells. The regions encased in black boxes are magnified. TM, trabecular meshwork; SC, Schlemm’s canal; red arrow indicates giant vacuoles.

Article Snippet: Following dissection, the TM or HTM cells grown on coverslips were immediately immersed in fresh transmission electron microscopy fixative (G1102; Servicebio, Wuhan, China) for 48 h. The samples then underwent post-fixation in a 1% OsO 4 aqueous solution for 2 h at room temperature.

Techniques: Control, Staining, Transmission Assay, Electron Microscopy

Journal: iScience

Article Title: Targeting Rap1-YAP1 mechanosignaling for ameliorating acute IOP elevation-induced trabecular meshwork dysfunction

doi: 10.1016/j.isci.2026.116268

Figure Lengend Snippet: Pathological changes in HTM cells under high hydrostatic pressure (A and B) Representative examples of human trabecular meshwork (HTM) cells cultured under control (con) or high hydrostatic pressure for 12 h, 1 day (1 D), and 3 days (3 D) immunolabeled for EDU (A) and TUNEL (B) assay. Scale bars, 50 μm. Bar graphs summarizing the effects of high hydrostatic pressure on the proliferation and death of HTM cells. N = 3. Magnification bars, 100 μm. (C) Histological evaluation of different groups of HTM cells by transmission electron microscopy. N = 3. Magnification bars, 50, 10, and 2 μm from the left to the right. (D) Representative fluorescence micrographs of F-actin in HTMs subjected to 12 h, 1D, and 3D culture under high hydrostatic pressure and the control, with nuclei and F-actin labeling shown in blue and green, respectively. The asterisk indicates cross-linked actin network (CLAN+) cells and the percentage of CLAN+ cells was analyzed. N = 5. Magnification bars, 25 μm. (E) Expression of fibronectin, collagen I and α-SMA in the different groups of HTM cells by western blots. N = 6. ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. Data represent mean ± SD.

Article Snippet: Following dissection, the TM or HTM cells grown on coverslips were immediately immersed in fresh transmission electron microscopy fixative (G1102; Servicebio, Wuhan, China) for 48 h. The samples then underwent post-fixation in a 1% OsO 4 aqueous solution for 2 h at room temperature.

Techniques: Cell Culture, Control, Immunolabeling, TUNEL Assay, Transmission Assay, Electron Microscopy, Fluorescence, Labeling, Expressing, Western Blot